Ornithine-containing lipids stimulate CD14-dependent TNF-α production from murine macrophage-like J774.1 and RAW 264.7 cells

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Abstract
The ornithine-containing lipids (OL)-induced cytokine production pattern in macrophage-like J774.1 and RAW 264.7 cells was different from that in the peritoneal macrophages previously reported. OLs, as well as lipopolysaccharide (LPS) of Escherichia coli, strongly induced tumor necrosis factor (TNF) a but not interleukin (IL)-1α in J774.1 cells. In the RAW cells, IL-1α, TNF-α and prostaglandin E2 were strongly induced by the OLs and LPS. OL- and serine—glycine-containing lipid (SGL)-induced TNF-α production in J774.1 and RAW 264.7 cells required serum. However, in CD14-deficient LR-9 cells, TNF-α was not induced by the OLs in the presence or absence of serum. OLs and a SGL almost completely inhibited the binding of 125I-LPS to J774.1 cells. These results suggested that OLs and SGL activate macrophages via the CD14-dependent pathway.