An indirect method for quantitation of cellular zinc content of timm-stained cerebellar samples by energy dispersive X-ray microanalysis
- 1 January 1988
- journal article
- research article
- Published by Springer Nature in Histochemistry and Cell Biology
- Vol. 89 (5) , 493-497
- https://doi.org/10.1007/bf00492607
Abstract
The absolute concentration of zinc in the Purkinje cells of the rat cerebellum was determined by means of energy dispersive X-ray microanalysis (EDAX). Gelatine blocks with known zinc concentrations were stained by Timm's sulphide-silver method, and their silver concentrations were measured by EDAX. A linear correlation was found between the zinc and silver concentrations and this linear function was used as a quantitative calibration for evaluation of sulphide-silver staining, after perfusion with sodium-sulphide solution, fixation with glutaraldehyde, cryostat sectioning and staining of cerebellar samples in Timm's reagent.Keywords
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