Enhanced Biological Activity of Antisense Oligonucleotides Containing 5-(1-Hexynyl)-substituted Pyrimidine Nucleotides
- 1 July 1997
- journal article
- Published by Taylor & Francis in Nucleosides and Nucleotides
- Vol. 16 (7-9) , 1717-1720
- https://doi.org/10.1080/07328319708006262
Abstract
Antisense oligonucleotides directed against HSV- 1 immediate early gene mRNA were synthesized with replacement of the internal pyrimidine nucleotides by the corresponding 5-(l-hexynyl) analogues and with end-capping by phosphorothioates. These compounds were found to have improved binding affinity, increased stability towards nucleases and enhanced antiviral activity in a cell culture assay.Keywords
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