Cloning and functional expression of a brain G‐protein‐coupled ATP receptor
- 14 June 1993
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 324 (2) , 219-225
- https://doi.org/10.1016/0014-5793(93)81397-i
Abstract
A cDNA encoding a novel member of the G-protein-coupled receptor (GCR) superfamily, an ATP receptor, has been isolated from an embryonic chick whole brain cDNA library by hybridization screening. The encoded protein has a sequence of 362 amino acids (41 kDa) and shares no more than 27% amino acid identity with any known GCR. When expressed as a complementary RNA (cRNA) in Xenopus oocytes a slowly-developing inward current was observed in response to application of ATP. The pharmacology of this expressed protein defines it as a P2Y purinoceptor.Keywords
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