HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC CHARACTERIZATION OF THE ROLE OF INORGANIC PYROPHOSPHATASE IN REGULATING THE REACTION OF URIDINE 5′-TRIPHOSPHATE WITH GLUCOSE 1-MONOPHOSPHATE

Abstract
Inorganic pyrophosphatase ( EC 3.6.1.1 ) catalyzing the hyrolysis of inorganic pyrophosphate drived the reaction of uridine 5′-triphosphate with glucose 1-monophosphate in the direction of uridine 5′-diphosphoglucose formation. The kinetic data in vitro supported the concept that inorganic pyrophosphate might be involved as one of products and its concentration level might regulate the above reaction in metabolic process.