Coprogen, A New Growth Factor Present in Dung, Required by Pilobolus Species

Abstract
SUMMARY A factor necessary for the growth of Pilobolus kleinii, P. longipes, P. umbonatus, and P. roridus has been demonstrated. This factor has been named Coprogen. A stock medium consisting of dung decoction and oatmeal extract has been described for carrying species of Pilobolus. A block inoculum assay has been developed for the assay of Coprogen in agar in Petri dishes. The pH for maximum growth has been shown to be above 7. Growth of Pilobolus kleinii in liquid media has been made possible resulting in a liquid assay that is both quantitative and qualitative. Certain aspects of spore germination have been studied in the genus Pilobolus.

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