The effect of unbalanced growth on recombinant formation inE. coli

Publisher Website
Google Scholar
Abstract
When zygotes formed by conjugation between Hfr and F- cells ofE. coliare transferred from an amino-acid rich medium to one lacking amino-acids the probability of genetic recombination is reduced. This result is interpreted as indicating that recombination can only occur during a limited period after zygote formation and that the metabolic imbalance caused by the change of medium leads to temporary inhibition of recombination. By an hour after zygote formation the yield of recombinants is no longer affected by transfer to amino-acid free medium.