Automated Vitamin C Analysis
Open Access
- 1 September 1972
- journal article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 18 (9) , 965-967
- https://doi.org/10.1093/clinchem/18.9.965
Abstract
An automated method of analysis for vitamin C (ascorbic acid, dehydroascorbic acid, diketogulonic acid) is described, in which ascorbic acid as oxidized to dehydroascorbic acid with Chloramine-T and determined by reaction with 2,4-dinitrophenylhydrazine. Results obtained by automated and manual methods agreed within 0.89% for ascorbic acid in liver. By the automated method, 96.7% of ascorbic acid added to tissue homogenates could be accounted for. The principal drawback to the proposed method is the use of 70% perchloric acid, a potent oxidizing agent, to solubilize the osazone.Keywords
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