Alteration by Diacylglycerols of the Transport and Fatty Acid Composition of Lymph Chylomicrons in Rats

Publisher Website
Google Scholar
Abstract
Lymph fistula rats were continuously infused with emulsions containing diacylglycerol consisting of 1,3-species (65.6%), 1(or 3),2-species (32.6%), and triacylglycerol (rapeseed oil) at the rate of 3ml/h for 1 h through a cannula inserted into the stomach. The lymph fluids were collected every hour for 5 h after starting the infusion of the lipid emulsions, and the lymph chylomicrons were isolated, purified and analyzed. Test emulsions were prepared to provide the same amount of fatty acids (144mg/h) as that in these acylglycerols. The rates for triacylglycerol transport at 2–3 h and for cholesterol transport by chylomicrons at 2–3 h and 3–4 h of the experimental period in rats infused with the diacylglycerol emulsion were significantly lower than the corresponding values in the rats infused with the triacylglycerol emulsion. As a consequence, the cumulative value for triacylglycerol transport at the end of the experimental period in rats infused with the diacylglycerol emulsion was significantly lower than the corresponding value in the rats infused with the triacylglycerol emulsion. In addition, cumulative values for cholesterol transport from 3h to the end of the experimental period were significantly lower in the former than in the latter. There was no difference in the total fatty acid compositon of chylomicron-triacylglycerol between the rats receiving the triacylglycerol and diacylglycerol emulsions. However, a considerable difference existed in the fatty acid composition at the 2-position of triacylglycerol between the two groups of rats. Thus, intragastric infusion of diacylglycerol mainly consisting of the 1,3-species compared to triacylglycerol not only altered the rate of lipid transport by lymph chylomicrons but also altered the structure of the triacylglycerol moiety in the rats.

This publication has 0 references indexed in Scilit: