Expression of Functional Fas Ligand in Choriocarcinoma

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Abstract
Hammer A, Hartmann M, Sedlmayr P, Walcher W, Kohnen G, Dohr G. Expression of functional Fas ligand in choriocarcinoma. AJRI 2002; 48:226–234 © Blackwell Munksgaard, 2002 PROBLEM: In the course of pregnancy, fetal trophoblast cells and in that of choriocarcinoma‐etiology, trophoblast derived tumor cells, invade the uterine mucosa without causing rejection by decidual leukocytes. Fas ligand (FasL, CD95L, APO‐1L), a central regulator of the immune system, has been implicated in the maintenance of immune privileged sites, such as the eye, the testis and the pregnant uterus by inducing apoptosis in activated infiltrating leukocytes. In normal pregnancy FasL, which is expressed by trophoblast cells, appears to contribute to the immune privilege of the pregnant uterus. As choriocarcinoma derives from trophoblast we wanted to assess the expression of FasL in this tissue. METHOD OF STUDY: Immunohistochemistry, immunofluorescence, TUNEL‐assay, Western blotting, coculture experiments and flourescence‐associated cell sorter‐analysis were the techniques used. RESULTS: Expression of FasL was found on cells of choriocarcinoma in paraffin sections in situ and on three choriocarcinoma cell lines such as JEG‐3, JAR and BeWo. These results were confirmed by Western blotting. In coculture experiments choriocarcinoma cells induced apoptosis in a Jurkat cell line – sensitive to FasL mediated killing. However, when the Jurkat cells were pre‐incubated with a Fas‐blocking monoclonal antibody, apoptosis was abolished to a great extent. CONCLUSION: Our findings show that choriocarcinoma cells express FasL and this aforementioned molecule is biologically active. We assume that FasL expression on choriocarcinoma cells may contribute to control of anti‐tumor responses by inducing apoptosis in activated Fas bearing leukocytes.