The Methylated Purines of Tetrahymena pyriformis Transfer Ribonucleic Acid*
- 1 August 1979
- journal article
- research article
- Published by Wiley in The Journal of Protozoology
- Vol. 26 (3) , 502-504
- https://doi.org/10.1111/j.1550-7408.1979.tb04660.x
Abstract
By phenol extraction and DEAE cellulose column chromatography, tRNA was isolated from T. pyriformis strain GL. Following acid hydrolysis of the tRNA, the methylated purine content was determined by Dowex 50 column chromatography and paper chromatography. The most abundant methylated guanine derivative was N2-DMG [N2-dimethylguanine]. Also present were 1-MG [1-methylguanine] N2-MG [N2-methylguanine] and 7-MG [7-methylguanine]. The most abundant methylated adenine was 1-MA [1-methyladenine]; no 2-MA (2-methyladenine) was detected. Small amounts of the N6-methyladenines were detected.Keywords
This publication has 14 references indexed in Scilit:
- Isolation and characterization of 2-methyladenosine from Escherichia coli tRNAGlu2, tRNAAsp1, tRNAHis1 and tRNAArgBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1972
- Small molecular weight RNA components in Tetrahymena pyriformisBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1971
- Methylated bases of ribosomal ribonucleic acid from HeLa cellsArchives of Biochemistry and Biophysics, 1968
- Methylated bases of transfer ribonucleic acid from hela and L cellsArchives of Biochemistry and Biophysics, 1968
- Properties of Soluble Ribonucleic Acid Methylases from Rat Liver*Biochemistry, 1967
- [37] Partition chromatography of nucleic acid components (Isolation of the minor nucleosides)Published by Elsevier ,1967
- Separation of soluble ribonucleic acid and of enzymes participating in protein synthesis and a study of specificity of these fractionsCollection of Czechoslovak Chemical Communications, 1966
- On the nucleic acids of green and colorless Euglena gracilis: Isolation and composition of deoxyribonucleic acid and of transfer ribonucleic acidBiochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects, 1962
- A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counterAnalytical Biochemistry, 1960
- Nucleotide composition of soluble ribonucleic acid from Escherichia coliJournal of Molecular Biology, 1960