Chorismate synthase. Pre-steady-state kinetics of phosphate release from 5-enolpyruvylshikimate 3-phosphate
- 1 February 1990
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 265 (3) , 899-902
- https://doi.org/10.1042/bj2650899
Abstract
The pre-steady-state kinetics of phosphate formation from 5-enolpyruvylshikimate 3-phosphate catalysed by Escherichia coli chorismate synthase (EC 4.6.1.4) were studied by a rapid-acid-quench technique at 25.degree.C at pH 7.5. No pre-steady-state ''burst'' or ''lag'' phase was observed, showing that phosphate is released concomitant with the rate-limiting step of the enzyme. The implications of this result for the mechanism of action of chorismate synthase are discussed.This publication has 9 references indexed in Scilit:
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