Detection of minor adducts in cisplatin‐modified DNA by transcription footprinting
- 24 May 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 323 (1-2) , 55-58
- https://doi.org/10.1016/0014-5793(93)81447-8
Abstract
Two DNA restriction fragments containing either a d(GC)5 or a d(TTGCTTGATTAGTTGTGTT) insert were subjected to reaction with cis-diamminedichloroplatinum(II) and were then used as templates for RNA synthesis by T7 RNA polymerase. Within the d(GC)5 insert, interstrand cross-links are preferentially formed. Within the second insert, the reactivity order of the potential binding sites is d(ApG) > d(GpC/ GpC) = d(GpA) > d(GpTpG). In the presence of cyanide ions, the adducts are much less stable at the d(GpA) sites than at the d(GpCpG) sites, in double-stranded DNAKeywords
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