Evaluation of the repliscan system for Enterobacteriaceae identification

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Abstract
A total of 1013 isolates of Enterobacteriaceae were identified in parallel by the Repliscan and API 20E systems. There was a 62% agreement at the genus level between the 2 systems. Of the 38% discrepant results, Repliscan classified 22% as biochemical pattern not on file, 8% as a multiple-genus group which included the API 20E identification and 8% as a genus other than that designated by API 20E. Relative to the various genera, Repliscan agreed with API 20E as follows: Escherichia coli, 80%; Klebsiella spp., 76%; Citrobacter spp., 75%; Proteus spp., 69%; Providencia spp., 54%; Serratia spp., 49%; Enterobacter spp., 25%; Shigella spp., 4% and Salmonella spp., 0%. Repliscan identified 35% of Enterobacter spp. isolates as Citrobacter spp., 91% of Shigella spp. isolates as a multiple-choice genus group, and 67% of Salmonella spp. isolates as biochemical pattern not on file. Repliscan agreed with API 20E at the species level as follows: E. coli, 80%; Klebsiella spp., 56%; Citrobacter spp., 66%; Proteus spp., 55%, Providencia spp., 46%, Serratia spp., 39%, Enterobacter spp., 18%; Shigella spp., 4% and Salmonella spp., 0%. The Repliscan system in its present stage of development does not reliably identify the Enterobacteriaceae.