Distribution of manganese in leaf tissues of manganese accumulator:Acanthopanax sciadophylloidesas revealed by Electronprobe X‐Ray Microanalyzer

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Abstract
The distribution of Mn in leaf tissues of a Mn accumulator: Acanthopanax sciadophlloides was investigated by means of electron‐probe X‐ray microanalysis using a scanning electron microscope in conduction with a cryostage. Unfixed and frozen plant specimens were used in this experiment. The results show that Mn is densely accumulated in the peripheral cells either of leaf, petiolule and petiole tissues. In the leaf tissues, Mn was highly concentrated in epidermis, palisade and spongy parenchyma cells. Considerable low Mn concentration was observed in vascular bundle cells. Distribution pattern of Mn in petiolule and petiole was similar and characteristic. In petiolule and petiole, most of the Mn was accumulated in the cell wall of epidermis, collenchyma and bundle sheath cells and least was in vascular bundle cells.