Increased Binding of [3H]GABA to Striatal Membranes Following Ischemia

Abstract
Sodium‐independent binding of [3H]γ‐aminobutyric acid ([3H]GABA) to membranes prepared from ischemic‐damaged rat striatum was studied by kinetic and time‐course analysis. Three days after 40 min of ischemia, [3H]GABA binding increased fourfold over control values. Scatchard analysis of the binding showed that ischemia significantly increased the affinity (KD) and the total number of binding sites (Bmax) for the high‐affinity GABA receptor. These results support the conclusion that transient forebrain ischemia damages striatal GABAergic neurons.