In vivo repair of the 3′ terminus of transfer RNA injected into amphibian oocytes
- 1 January 1977
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 4 (6) , 1873-1880
- https://doi.org/10.1093/nar/4.6.1873
Abstract
Yeast transfer RNA specific for phenylalanine has been treated chemically to remove either one or two nucleotides of its 3' terminus and has been injected into Xenopus laevis oocytes to test whether this RNA can be repaired in vivo. The results obtained showed that oocytes could aminoacylate and thus repair tRNAPhe that has lost both its terminal adenosine and 3' phosphate. A similar result was obtained with tRNAPhe that had undergone two full cycles of 3' terminal nucleotide removal. The oocytes cannot aminoacylate tRNAPhe whose 3' terminal ribose has been oxidized with periodate or the derivative that retains a 3' phosphate after adenosine removal. In vitro assays show that the Xenopus ovary contains a tRNA nucleotidyl transferase with the properties similar to enzymes obtained from other sources which may be responsible for the 3' terminal repair observed in vitro.Keywords
This publication has 11 references indexed in Scilit:
- Changes in tRNA nucleotidyltransferase activity during embryonic development of Xenopus laevisExperimental Cell Research, 1976
- Aminoacylation of transfer RNA microinjected into Xenopus laevis oocytesNature, 1975
- Transfer Ribonucleic Acid Nucleotidyl-transferase Plays an Essential Role in the Normal Growth of Escherichia coli and in the Biosynthesis of Some Bacteriophage T4 Transfer Ribonucleic AcidsJournal of Biological Chemistry, 1974
- The degradation of ribonucleic acids injected into xenopus laevis oocytesCell, 1974
- Synthesis and Functions of the -C-C-A Terminus of Transfer RNAProgress in Nucleic Acid Research and Molecular Biology, 1973
- Translation of Encephalomyocarditis Viral RNA in Oocytes of Xenopus laevisProceedings of the National Academy of Sciences, 1972
- Determination of the nucleoside triphosphate contents of eggs and oocytes of Xenopus laevisBiochemical Journal, 1972
- Reactions at the 3' terminus of transfer ribonucleic acid. 3. Catalytic properties of two purified rabbit liver transfer ribonucleic acid nucleotidyl transferases.1972
- Rabbit haemoglobin synthesis in frog cells: the translation of reticulocyte 9 s RNA in frog oocytesJournal of Molecular Biology, 1971
- Enzymic incorporation of ribonucleotides and amino acids into transfer ribonucleic acidJournal of Molecular Biology, 1965