RELATION OF CFU-C PRESERVATION TO THE FREEZING RATE FOLLOWING THE HEAT OF FUSION

  • 1 January 1982
    • journal article
    • research article
    • Vol. 294  (2) , 103-106
Abstract
Bags of human bone marrow (71) cryopreserved and stored for periods up to 2 yr were thawed and CFUc [pluripotent stem cell] recoveries were studied in relation to the rate of freezing following the heat of fusion. Excellent CFUc recoveries (> 50%) were obtained in all cases (11/11) for freezing rates < 5.degree. C/min, whereas freezing rates > 10.degree. C/min produced poor recoveries in 78% of the cases (4/18). Intermediate freezing rates (5 < .theta. < 10) resulted in intermediate yields of CFUc, with good recoveries in only 45% of the cases (19/42). CFUc recoveries and, subsequently, the kinetics of autologous engraftment following the infusion of cryopreserved marrow, which are already known to be related both to the freezing rate before the heat of fusion and the duration of the phase transition period, are evidently related to this new parameter, which should be carefully monitored during the freezing procedure.