Evaluation of Ciliary Movement in Tracheal Rings to Assess Immunity against Infectious Bronchitis Virus

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Abstract
Chickens vaccinated at 1 and 14 days of age with a live vaccine containing the Massachusetts and Connecticut strains of infectious bronchitis virus were protected against challenge by the Massachusetts strain administered at 5 wk. The virus recovery method in which tracheal swabs taken 4 days post-challenge are used to inoculate chicken embryos was compared with the method based on ciliary activity in tracheal explants. The evaluation of ciliary activity effectively measured immunity to infectious bronchitis virus. Serological results obtained in a microneutralization test using chicken embryo kidney cells correlated with the immune status of the birds and the .alpha. neutralization results obtained using chicken embryos.