Purification Of Methionine Acceptor tRNA On Arginine-Agarose

Abstract
Crude E. coli tRNA or enriched methionine acceptor tRNA can be separated into three stiecies on a column of arginine-agarose. The first peak eluted is tRNAMet and the latter two peaks are two forms of tRNAMet f. From crude tRNA, tRNAMet m is obtained in approximately 50% purity. Arginine-agarose separates enriched methionine accepting tRNA into three homogeneous fractions.