Direct and indirect effects of antibiotics on granulocyte activity

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Abstract
Tetracyclines have been shown to depress granulocyte migration, phagocytosis and adherence to glass. Two β -lactam antibiotics, ampicillin and a new third generation cephalosporin ceftazidime were investigated for their direct influence on granulocyte migration. They were found to have no deleterious effect on the cell migration response. Subminimal inhibitory concentrations of ampicillin and ceftazidime, incubated with growing cultures of Escherichia coli R987, were found to cause an increased stimulation of granulocyte migration response when culture filtrates were used as chemotactic factors in the LMAT (leukocyte migration under agarose technique). A significant stimulation was obtained only from the cephalosporin derivative. Doxycyline, investigated in the same manner, did not evoke any increased migratory response. The different effects of antibiotic release or leakage of chemotactic factors from E. coli were most probably due to the different modes of action on the bacterial cell walls.

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