A procedure for the assay of DNA damage in mammalian cells by alkaline elution and microfluorometric DNA determination.

  • 1 November 1977
    • journal article
    • research article
    • Vol. 26  (6) , 419-27
Abstract
The microfluorimetric assay of Kissane and Robins (J. Biol. Chem. 233, 184-188, 1958) has been modified to monitor DNA content in alkaline elution fractions. The elution profiles of DNA from treated and control cultured cells demonstrated a good correlation whether DNA in the elution fractions was determined by the fluorometric method or by measuring its radioactivity. The DNA recovery was of about 80% and the measured material was DNase digestible.

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