Serial Analysis of Gene Expression
- 20 October 1995
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 270 (5235) , 484-487
- https://doi.org/10.1126/science.270.5235.484
Abstract
The characteristics of an organism are determined by the genes expressed within it. A method was developed, called serial analysis of gene expression (SAGE), that allows the quantitative and simultaneous analysis of a large number of transcripts. To demonstrate this strategy, short diagnostic sequence tags were isolated from pancreas, concatenated, and cloned. Manual sequencing of 1000 tags revealed a gene expression pattern characteristic of pancreatic function. New pancreatic transcripts corresponding to novel tags were identified. SAGE should provide a broadly applicable means for the quantitative cataloging and comparison of expressed genes in a variety of normal, developmental, and disease states.Keywords
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