Methods For Removing Uranyl Acetate Precipitate From Ultrathin Sections

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Abstract
Precipitate resulting from en bloc staining with uranyl acetate [for transmission electron microscopy] was removed by treating sections [of Aedes aegypti midgut] with 15% oxalic acid in 50% methanol for 30 min at 40.degree. C. Precipitate resulting from poststaining sections with hot uranyl acetate was removed by rinsing sections in 0.25-0.50% aqueous oxalic acid for 10-15 s at room temperature. Rinsing sections for longer than 30 s removed uranyl precipitate and also destained the sections. These procedures did not damage the embedding medium or cellular detail.