Effect of tunicamycin on the metabolism of low-density lipoproteins by control and low-density-lipoprotein-receptor-deficient human skin fibroblasts
- 14 January 1980
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 186 (1) , 373-375
- https://doi.org/10.1042/bj1860373
Abstract
Preincubation of normal human skin fibroblasts with tunicamycin, which inhibits N-glycosylation of glycoproteins, resulted in a dose-dependent and reversible inhibition of binding and internalization of homologous low-density lipoproteins by the cells. The degradation of the internalized lipoproteins was not affected by the drug. Comparative studies with fibroblasts deficient in low-density-lipoprotein receptors indicated that tunicamycin exerts its inhibitory effect only via the receptor-mediated high-affinity binding and uptake of lipoproteins. Expression of low-density-lipoprotein receptors on the cell surface of human skin fibroblasts apparently depends on intact N-glycosylation.This publication has 17 references indexed in Scilit:
- Role of net charge of low density lipoproteins in high affinity binding and uptake by cultured cellsBiochemical and Biophysical Research Communications, 1979
- Sialic‐Acid Content of Low‐Density Lipoproteins Controls Their Binding and Uptake by Cultured CellsEuropean Journal of Biochemistry, 1979
- Release of low density lipoprotein receptors from human fibroblasts or Hela cells by tryptic digestionBiochemical and Biophysical Research Communications, 1978
- Role of carbohydrates in protein secretion and turnover: Effects of tunicamycin on the major cell surface glycoprotein of chick embryo fibroblastsCell, 1978
- Changes in surface properties of normal and transformed cells caused by tunicamycin, an inhibitor of protein glycosylation.Proceedings of the National Academy of Sciences, 1977
- Regulation of lipid synthesis by low density lipoproteins in cultured skin fibroblasts in homozygous familial hypercholesterolemiaBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1977
- Evidence for the participation of saccharide-lipids in the synthesis of the oligosaccharide chain of ovalbumin.Journal of Biological Chemistry, 1977
- Regulation of the activity of the low density lipoprotein receptor in human fibroblastsCell, 1975
- Tunicamycin inhibition of polyisoprenyl N-acetylglucosaminyl pyrophosphate formation in calf-liver microsomesBiochemical and Biophysical Research Communications, 1975
- Nigrosin as a dye for differentiating live and dead ascites cellsExperimental Cell Research, 1958