Expression of human immunoglobulin E ε chain cDNA inE.coli
- 25 May 1983
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 11 (10) , 3077-3085
- https://doi.org/10.1093/nar/11.10.3077
Abstract
Using the cDNA of human 1D6DC; chain, three expression plasmids that code directly the constant portion of the 1D6DC; chain (C1D6DC;l-C 1D6DC;4, C 1D6DC;2-C 1D6DC;C4 and C 1D6DC;3-C 1D6DC;4 domains) were constructed. These 1D6DC; chain peptides were synthesized in E. coli under the control of the trp promoter-operator. The bacterially produced peptides have the antigenicity of human 1D6DC; chain and gave the molecular weights equal to the values calculated from the amino acid sequence of the constructed plasmidsKeywords
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