Possible Etiological Rôle of Plasmodiophora tabaci in Tobacco Mosaic

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Abstract
P. tabaci can be cultivated from both healthy, plants and those affected with mosaic, provided they are not washed in an antiseptic solution such as HgCl2. If washed in HgCl2, P. tabaci frequently can be obtained from diseased, but only rarely from healthy plants. Inoculation of tobacco plants with cultures containing various stages of P. tabaci is followed by mosaic only when the cultures are derived from diseased plants, in which case a concomitant mosaic virus could be present and carried by dilution. Cultures prepared from diseased leaves are sometimes negative for P. tabaci but still able to produce mosaic. Filtrates from diseased plants are infective but do not show P. tabaci after standing for various periods of time. Furthermore, such filtrates are suitable culture media for P. tabaci, as was demonstrated at the end of each experiment. P. tabaci in the plasmodial stage does not pass diatomaceous or porcelain filters.

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