Compartmentation of deoxypyrimidine nucleotides for nuclear DNA replication in S phase mammalian cells
- 1 September 1989
- journal article
- research article
- Published by Wiley in Journal of Molecular Recognition
- Vol. 2 (2) , 75-83
- https://doi.org/10.1002/jmr.300020205
Abstract
DNA synthesis in S phase Chinese hamster embryo fibroblast cells in the presence of exogenous 3H-dUrd shows incorporation of the labeled precursor with very little dilution by the large unlabeled intracellular precursor pools. Full mixing would predict a specific activity 10-fold less than that measured. This coupled with the finding that 80% of the radioactivity from the exogenous 3H-dUrd appears in the karyoplasts implies a compartmentation where 3H-dUMP and 3H-dTTP derived from exogenous 3H-dUrd do not mix freely with endogenous cytoplasmic pools.Keywords
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