A Proteoglycan Related to the Urinary Trypsin Inhibitor (UTI) Links the two Heavy Chains of Inter-α-Trypsin Inhibitor

Publisher Website
Google Scholar
Abstract
CDNA studies have suggested that inter-.alpha.-trypsin inhibitor (ITI) is a complex of several different peptide chains; the sequence of the inhibitory part of ITI is in excellent agreement with that of the urinary trypsin inhibitor (UTI). The present report demonstrates that a compound immunologically related to UTI is released by digestion with porcine pancreatic elastase or human leucocyte elastase. Since UTI has been shown to be a proteoglycan, ITI has been treated by chondroitinase. In these conditions, ITI is dissociated and gives rise to two heavy chains (78 and 85 kDa) and one light chain (26 kDa) immunologically related to UTI and which PAGE moves close to UTIc (produced by chondroitinase treatment of URI) we suggest that ITI is non-covalent complex comprising two heavy chains and one light chain immunologically related to UTI and which is also a proteoglycan.