Brain α‐dystroglycan displays unique glycoepitopes and preferential binding to laminin‐10/11
- 11 May 2006
- journal article
- Published by Wiley in FEBS Letters
- Vol. 580 (14) , 3381-3385
- https://doi.org/10.1016/j.febslet.2006.05.010
Abstract
α‐Dystroglycan was quantitatively enriched from mammalian brain based on its uniform reactivity with Vicia villosa agglutinin and resolved into sub‐populations possessing or lacking the sulfated glucuronic acid epitope recognized by monoclonal antibody HNK‐1. We generated a new monoclonal antibody specific for a glycoepitope on brain α‐dystroglycan but absent from α‐dystroglycan expressed in all other tissues examined. Finally, we found that laminin‐10/11 preferentially bound to brain α‐dystroglycan compared to skeletal muscle α‐dystroglycan. Our results suggest that tissue‐specific glycosylation modifies the laminin binding specificity of α‐dystroglycan.Keywords
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