Microscale production of hybridomas by hypo-osmolar electrofusion

1 March 1992

journal article
Published by SAGE Publications in Human Antibodies

Vol. 3 (1) , 14-18
https://doi.org/10.3233/hab-1992-3103

Abstract

We have developed and tested a novel electrofusion chamber, the adjustable plate microchamber, that permits the successful electrofusion and production of hybridomas in a hanging droplet from as few as 1,000 B lymphocytes. Cell suspension volumes of

Keywords

LYMPHOCYTES
PERMITS
MICROCHAMBER
PRODUCTION OF HYBRIDOMAS
HYPO OSMOLAR
OSMOLAR ELECTROFUSION

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