Assessment of Cytoplasmic Contaminations in Isolated Vacuole Preparations
Open Access
- 1 January 1980
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 65 (1) , 85-87
- https://doi.org/10.1104/pp.65.1.85
Abstract
A method for testing the purity of isolated vacuoles is proposed. Preparations of isolated vacuoles often contain “impure vacuoles,” namely, vacuoles contaminated with some cytoplasmic remnants. Such impure vacuoles are stained by the vital fluorescent dye fluorescein-diacetate. Vacuoles enclosed only by the tonoplast, “naked vacuoles,” do not stain.This publication has 3 references indexed in Scilit:
- Isolation of Vacuoles from Root Storage Tissue of Beta vulgaris L.Plant Physiology, 1976
- Purification and viability determinations of plant protoplastsPlanta, 1976
- Membrane properties of living mammalian cells as studied by enzymatic hydrolysis of fluorogenic esters.Proceedings of the National Academy of Sciences, 1966