Micropropagation of Capparis decidua (Forsk.) Edgew. — a tree of arid horticulture

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Abstract
A method for micropropagation of mature trees of Capparis decidua was developed. Multiple shoots were obtained from nodal explants on Murashige and Skoog's (1962) medium+0.1mgl−1 NAA+5.0mgl−1BAP+additives (50mgl−1 ascorbic acid and25 mgl−1 each of adenine sulphate, L-arginine and citric acid) at 28 ± 2°C, 12 h/dphotoperiod and 35–40 μmol m-2s−1 photon flux density. The shoots were multiplied by (i) subculture of nodal shoot segments onto MS +0.1 mgl-−1 IAA+1.0mgl−1 BAPH+additives, and (ii) repeated transfer of original explant onto MS+ 0.1mgl−1 IAA+mg l−1 BAP+additives, at intervals of 3 weeks. Sixty to 70% of the shoots rooted when pulse treated with 100 mg l−1 IBA in half strength MS liquid medium for 4h, and then transferred onto hormone-free half-strength agar-gelled MS basal saltmedium. Incubation in dark at 33 ± 2°C for 6d favoured root induction. In vitro hardened plants were transferred to pots.

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