A Separation Study of Tricyclic Antidepressant Drugs by HPLC with β-Cyclodextrin Bonded Stationary Phase
- 1 October 1993
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 16 (16) , 3487-3508
- https://doi.org/10.1080/10826079308019703
Abstract
A systematic study of the separation of a series of tricyclic antidepressant drugs (amitriptyline, nortriptyline, protriptyline, maprotiline, imipramine, chlori-pramine, doxepin-E and doxepin-Z) using reversed phase HPLC with β-cyclodextrin (β-CD) bonded stationary phase is presented. The effect of the organic modifier (methanol) content and pH value of the aqueous phase of the mobile phase on retention was examined and experimental equations of these dependences were derived.The separation is mainly due to the variations of the strength of the CD-solute complexes and this was proved by the good relationship found experimentally between corrected logk' (referred to pure aqueous mobile phase) and the log of complex formation constants. Using the chromatographic response function optimization procedure a mobile phase of 50/50 methanol/buffer pH 6.5 was found as optimum for separations of analytical use. The elution order of the solutes was tried to be correlated to various structure differences.Keywords
This publication has 29 references indexed in Scilit:
- Solution studies of .beta.-cyclodextrin-pyrene complexes under reversed-phase liquid chromatographic conditions: effect of alcohols as mobile-phase comodifiersAnalytical Chemistry, 1991
- A Study of the Solvent Composition Effects on the Separation of Seven Clinically Important Porphyrins on Cyclodextrin Bonded PhasesJournal of Liquid Chromatography, 1990
- High-performance liquid chromatographic resolution of racemic 1,4-benzodiazepin-2-ones by means of a β-cyclodextrin silica bonded chiral stationary phaseJournal of Chromatography A, 1990
- Evaluation of the effect of organic modifier and pH on retention and selectivity in reversed-phase liquid chromatographic separation of alkaloids on a cyclodextrin bonded phaseAnalytical Chemistry, 1990
- Enantiomeric resolution and chiral recognition of racemic nicotine and nicotine analogs by .beta.-cyclodextrin complexation. Structure-enantiomeric resolution relationships in host-guest interactionsAnalytical Chemistry, 1988
- The Multimodal Cyclodextrin Bonded Stationary Phases for High Performance Liquid ChromatographyJournal of Liquid Chromatography, 1988
- Separation of Metallocene Enantiomers by Liquid Chromatography: Chiral Recognition Via Cyclodextrin Bonded PhasesAnalytical Chemistry, 1985
- Liquid chromatographic separation of enantiomers using a chiral .beta.-cyclodextrin-bonded stationary phase and conventional aqueous-organic mobile phasesAnalytical Chemistry, 1985
- Liquid chromatographic separation of diastereomers and structural isomers on cyclodextrin-bonded phasesAnalytical Chemistry, 1985
- Simultaneous liquid Chromatographie analysis of amitriptyline, nortriptyline, imipramine, desipramine, doxepin, and nordoxepinClinica Chimica Acta; International Journal of Clinical Chemistry, 1981