Electron microscopy of hybridoma cells with special regard to monoclonal antibody production
- 1 July 1990
- journal article
- conference paper
- Published by Springer Nature in Cytotechnology
- Vol. 4 (1) , 13-28
- https://doi.org/10.1007/bf00148807
Abstract
Electron microscopy of mouse hybridoma cell lines shows that the major difference between non, low and high producer cell lines is the amount of endoplasmic reticulum. Vesicular-tubular or cavernous structures of endoplasmic reticulum, which can survive long after cell death, are particularly abundant in producer cell lines. Immunogold labelling with anti-mouse IgG reveals that antibodies are predominantly located in these structures. The cell membrane undergoes structural changes during the late stages of batch culture with the disappearance of microvilli and the appearance of blebs and deep indentations. Necrosis disrupts the cytoplasmic structures and the nucleus is last to degrade.This publication has 4 references indexed in Scilit:
- Intracellular degradation of newly synthesized secretory proteinsBiochemical Journal, 1983
- Cell death: a new classification separating apoptosis from necrosisPublished by Springer Nature ,1981
- The ageing, growth and death of cellsNature, 1974
- Uropod formation in phytohaemagglutinin (PHA) stimulated lymphocytesExperimental Cell Research, 1971