The Reaction of Immunoglobulin-Coated Bentonite and Radioiodinated Antigen as a Basis for Antibody Detection and Antigen Binding

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Abstract
A colloidal suspension of bentonite particles prepared on the basis of differential sedimentation was found to adsorb irreversibly about its own weight of rabbit immunoglobulin G. The immunoglobulin-coated bentonite formed a colloidal suspension readily in normal rabbit serum and easily sedimented upon centrifugation. Thus, from a reaction mixture of adsorbed antibody and a radioactively-labeled antigen, the separation of free and bound antigen can be accomplished by centrifugation. When the adsorbed antibody to an albumin antigen was used, the antigen-binding characteristics of the adsorbed antibody were found to be essentially similar to those of the free or unadsorbed antibody as demonstrated by the Farr method; however, the Farr method was two to four times more sensitive in estimating antigen-binding. It is suggested that the present method of using inert particles coated with antibodies for detecting these antibodies may be extended to the detection of antibodies of other immunoglobulin classes or of antibodies in antiserum.

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