Purification and characterization of Clostridium perfringens iota toxin: dependence on two nonlinked proteins for biological activity

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Abstract
Clostridium perfringens type E iota toxin, a dermonecrotic and lethal binary toxin, was purified to homogeneity. Each protein component of the toxin, iota a (ia) or iota b (ib), appeared as a single band by gradient or sodium dodecyl sulfate-polyacrylamide gel electrophoresis and yielded a single immunoprecipitin arc by crossed immunoelectrophoresis with homologous antiserum. Individually, ia (Mr 47,500) or ib (Mr 71,500) had little biological activity. However, when combined in equimolar amounts, there was a 64-fold increase in the guinea pig dermonecrotic titer. The biological activity of ia was heat stable (85.degree. C for 15 min), whereas ib was inactivated at 55.degree. C. Our results demonstrated that C. perfringens iota toxin required two different, nonlinked protein components for biological activity.