Some Considerations in Agar Column Diffusion Analyses

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Abstract
Summary: By direct photometry the zone density of human serum albumin homologous systems diffusing in agar columns varied inversely with the initial antigen concentrations up to 150 hr reaction time. Such observation is a corollary to the general rule that the antibody concentration alone governs the zone density. Nonspecific substances added to human serum albumin increased the zone densities; whereas the removal of albumin from rabbit antiserum decreased zone densities. This effect is probably due to altered solubilization of the precipitates. Micro-Kjeldahl analyses of whole diffusion patterns containing several zones will provide an absolute measure for indicating the reactivity of a complex precipitin system. Such measure is not presently obtainable by the classic quantitative precipitin method.

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