LIPID STAINING ON SEMITHIN SECTIONS WITH SUDAN BLACK B OR NILE BLUE SULPHATE. APPLICATION TO INTESTINAL FAT ABSORPTION

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Abstract
The specific characterization of lipids was investigated by lysochromes on routine semithin sections of osmium-fixed tissues embedded in epon 812 for ultrastructural study. The work was done on trout and rat intestine during fat absorption. The best results were obtained after prefixation with paraformaldehyde; 1-1.5 .mu.m sections were stained by Sudan Black B and Nile Blue sulfate under well defined conditions. The use of H2O2 makes staining by lysochromes possible because: it eliminates the primary blackness due to osmium fixation and allows the visualization of lysochromes which are dissolved in the lipids; it increases the porosity of resin and allows the excess of lysochromes into the tissue lipids. The results obtained with Nile blue sulfate (coloring blue and red lysochrome) are discussed. A counterstaining with Ehrlich hematoxylin can be performed for later morphological study. A further ultrastructural study allowed the identification of stained structures by Sudan Black B and Nile blue sulfate.