A Dose-response Study on Opening of Imidazole Ring of Adenine in DNA by Ionizing Radiation

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Abstract
A dose-response relationship between .gamma.-irradiation and the cleavage of the imidazole ring of adenine in [Bacillus subtilis] in DNA to form formamidopyrimidine was demonstrated. When the DNA aqueous solution was irradiated with 0.1 Gy [Gray] under N2O, there is little evidence of imidazole ring cleavage. A significant increase in cleavage begins to be noticed above 1 Gy reaching a plateau at 1000 Gy. No formamidopyrimidine was formed when 2''-deoxyadenosine was irradiated with up to 1000 Gy. A dose of 100 Gy converts 18% of adenine in DNA to formamidopyrimidine. In irradiated DNA aqueous soluton 1000 Gy convert 25% of adenine to formamidopyrimidine under N2O. Some of the adenine was converted to 7,8-dihydro-8-oxoadenine but in amount that is 20% of that converted to formamidopyrimidine under N2O. There was more adenine in DNA converted to formamidopyrimidine under N2O than under N2.

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