Comparison of Cultureset and primary rabbit kidney cell culture for the detection of herpes simplex virus

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Abstract
Herpes simplex virus isolation by conventional cell culture in primary rabbit kidney cells (RK) was compared with Cultureset (CS). At 24 h CS was more sensitive (31 versus 25 of 55 isolates detected) than RK, but at 48 h there was little difference (43 detected by CS, 46 by RK). CS stained at 48 h was significantly less sensitive (78.2%) than RK observed for 1 week. CS stained at 24 or 48 h was not an acceptable substitute for RK observed for 1 week.