EVIDENCE FOR A SPECIFIC PROTEIN ASSOCIATED TO THE PLASMA-MEMBRANE OF CHOLINERGIC SYNAPTOSOMES

  • 1 January 1982
    • journal article
    • research article
    • Vol. 78  (4) , 433-442
Abstract
Synaptosomal plasma membrane fractions were prepared by fractionation of pure Torpedo cholinergic synaptosomes. A 67,000 dalton peptide was a major component of the presynaptic membrane. It appears specific for this membrane since it copurifies with the synaptosomal plasma membrane; it was not present in similar plasma membranes but prepared from Torpedo electric lobes or electric nerves; and since rabbit antibodies to presynaptic antigens which were mainly directed to this 67,000 dalton peptide band were shown to bind to the nerve terminal network in Torpedo electric organ. A crude fraction of presynaptic plasma membrane prepared from electric organ homogenate could be a convenient material for the purification of this 67,000 dalton peptide.

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