Optimized β-galactosidase staining method for simultaneous detection of endogenous gene expression in early mouse embryos
- 10 February 2006
- Vol. 44 (2) , 57-65
- https://doi.org/10.1002/gene.20186
Abstract
β‐Galactosidase (β‐gal) is one of the popular reporters for detecting the expression of endogenous or exogenous genes. Here we report 6‐chloro‐3‐indoxyl‐beta‐D‐galactopyranoside (S‐gal) is more sensitive for β‐gal activity than 5‐bromo‐4‐chloro‐3‐indolyl‐beta‐D‐galactoside (X‐gal), particularly during the early developmental stages of mouse embryos. Further, we successfully combined β‐gal staining with S‐gal and in situ hybridization using DIG‐labeled probes in both whole and sections of early stage embryos due to the sensitivity and color compatibility of S‐gal. genesis 44:57–65, 2006.Keywords
This publication has 13 references indexed in Scilit:
- BMP signaling through ACVRI is required for left–right patterning in the early mouse embryoDevelopmental Biology, 2004
- A specific requirement for PDGF-C in palate formation and PDGFR-α signalingNature Genetics, 2004
- Conditional inactivation ofTgfbr2in cranial neural crest causes cleft palate and calvaria defectsDevelopment, 2003
- Spatial and temporal ‘knock down’ of gene expression by electroporation of double-stranded RNA and morpholinos into early postimplantation mouse embryosMechanisms of Development, 2002
- Epiblast-restricted Cre expression in MORE mice: A tool to distinguish embryonic vs. extra-embryonic gene functionGenesis, 2000
- Multiple Roles for Activin-Like Kinase-2 Signaling during Mouse EmbryogenesisDevelopmental Biology, 1999
- A Novel Transgenic Technique That Allows Specific Marking of the Neural Crest Cell Lineage in MiceDevelopmental Biology, 1999
- Disruption of overlapping transcripts in the ROSA βgeo 26 gene trap strain leads to widespread expression of β-galactosidase in mouse embryos and hematopoietic cellsProceedings of the National Academy of Sciences, 1997
- In situ hybridization and β-galactosidase: a powerful combination for analysing transgenic miceTrends in Genetics, 1995
- Proneural clusters of achaete-scute expression and the generation of sensory organs in the Drosophila imaginal wing disc.Genes & Development, 1991