A Rapid and Reproducible Hcmogenization Procedure for the Isolation of Plasma Membranes from Rat Liver

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Abstract
A simplified modification of the Neville procedure for the isolation of plasma membranes from rat liver is described in which cells are broken by low-shear homogenizetion with a Polytron homogenizer. Plasma membranes are recovered from the homogenates by differential and discontinuous sucrose gradient centrifugetions. The procedure provides plasma membrane fractions enriched 25-fold for AMPase, a marker enzyme for the plasma membrane of rat liver, with a combined contamination from endoplasmic reticulum, Golgi apparatus and mitochondria of less than 10% The procedure is uncomplicated, reproducible, and yields enzymatically active plasma membrane fractions of high purity.

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