Identification of Two Herpes Simplex Virus Type 1-induced Proteins (21K and 22K) which Interact Specifically with the a Sequence of Herpes Simplex Virus DNA
- 1 September 1984
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 65 (9) , 1467-1475
- https://doi.org/10.1099/0022-1317-65-9-1467
Abstract
A DNA competition binding assay was used to search for herpes simplex virus (HSV) proteins which bind to specific sequences of the genome of HSV. Cloned DNA from different regions of the virus genome were tested. Two late polypeptides, one major of apparent MW 21,000 (21K) and one minor of 22,000 (22K), were preferentially bound by a variety of fragments containing the HSV-1400 base-pair (bp) a sequence (a direct repeat present at the ends of the molecule and in inverted orientation between the long and short regions of the genome) but not by other competing DNA including ones containing an origin of replication. Evidently the HSV type 1-induced 21K and 22K polypeptides interact specifically with DNA sequences within this 400 bp HSV-1 a sequence.This publication has 3 references indexed in Scilit:
- Thermolabile in vivo DNA-binding activity associated with a protein encoded by mutants of herpes simplex virus type 1Journal of Virology, 1983
- Recombination and linkage between structural and regulatory genes of herpes simplex virus type 1: study of the functional organization of the genomeJournal of Virology, 1980
- Sequence arrangement in herpes simplex virus type 1 DNA: identification of terminal fragments in restriction endonuclease digests and evidence for inversions in redundant and unique sequencesJournal of Virology, 1976