Molecular cloning of a .BETA.-xylosidase gene from Bacillus subtilis.
- 1 January 1985
- journal article
- research article
- Published by Microbiology Research Foundation in The Journal of General and Applied Microbiology
- Vol. 31 (6) , 513-518
- https://doi.org/10.2323/jgam.31.513
Abstract
The .beta.-D-xylosidase gene (xynB) from Bacillus subtilis PAP115 was cloned via the pBR325 vector in Escherichia coli. The resulting hybrid plasmid named pRH300, contained a 4.6 kbp EcoRI-bordered insert. A subcloning procedure, using the pUC8 plasmid, allowed the determination of the location of the xynB gene of a 3.18 kbp fragment which was cloned into the pRH271 plasmid (a pBR325 vector harboring the xylanase gene (xynA) from B. subtilis PAP1115). The new vector, named pRH100, enabled E. coli to produce an intracellular xylanase and a cell-bound xylosidase.Keywords
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