The recovery of Arcobacter butzleri NCTC 12481 from various temperature treatments

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Abstract
Aims: The aim of this study was to determine the growth and survival characteristics for Arcobacter butzleri NCTC 12481. Methods and Results: The temperature and pH growth ranges were 15–39°C and pH 6·0–8·0, as determined using impedance microbiology. The maximum specific growth rate was 00·57 h–1 at 30°C, pH 7·0. Arcobacter butzleri harvested from the exponential phase was more resistant to heat treatment than stationary phase cells (D55 1·1 and 0·4 min, respectively). Fluorescent dye uptake, and the release of UV-absorbing material, increased in heat-treated cells. After 21 d storage at 4 and –20°C, A. butzleri was recovered on blood agar, but not on the isolation media CAT or CCDA. Conclusion:Arcobacter butzleri cells from the exponential phase were less heat sensitive than those from the stationary phase. The organism was able to survive cold storage for at least 3 weeks. Significance and Impact of the Study: The growth and survival characteristics have been quantified thus providing a greater understanding of this newly emerging pathogen.