Structural Heterogeneity in DNA: Temperature Dependence of 2‐Aminopurine Fluorescence in Dinucleotides
- 5 August 2005
- journal article
- research article
- Published by Wiley in Chemphyschem
- Vol. 6 (8) , 1622-1627
- https://doi.org/10.1002/cphc.200400648
Abstract
The fluorescent base analogue 2‐aminopurine is a sensitive probe for local dynamics of DNA. Its fluorescence is quenched by interaction with the neighboring bases, but the underlying mechanisms are still under investigation. We studied 2‐aminopurine fluorescence in dinucleotides with each of the natural bases. Consistently, two of the four fluorescence‐decay components depend strongly on temperature. Our results indicate that these components are due to the excited‐state dynamics of a single conformational state. We propose a variation of the gating model in which transient unstacking occurs in the excited state.Keywords
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