DNA-fluorometry of mammalian sperm
- 1 January 1977
- journal article
- research article
- Published by Springer Nature in Histochemistry and Cell Biology
- Vol. 54 (1) , 1-7
- https://doi.org/10.1007/bf00493324
Abstract
The DNA-content of sperm and testicular cells was measured by pulse-cytophotometry with high resolution. From flat sperm symmetric and narrow fluorescence distributions were obtained. Enzymatic treatment with papain or pronase and staining with an ethidiumbromide-mithramycin dye solution generate stoichiometric DNA-staining including that of mature sperm with a coefficient of variation below 2%.Keywords
This publication has 5 references indexed in Scilit:
- Flow microfluorometric analysis of sperm DNA content: Effect of cell shape on the fluorescence distributionJournal of Cellular Physiology, 1976
- Durchflußfluorescenzcytophotometrie für ultraschnelle DNS-Messungen an großen ZellpopulationenHistochemistry and Cell Biology, 1971
- Changes in deoxyribonucleoprotein during spermiogenesis in the bullExperimental Cell Research, 1966
- Synthetic activities during spermatogenesis in the mouseExperimental Cell Research, 1965
- Autoradiographic evidence of a nuclear histone synthesis during mouse spermiogenesis in the absence of detectable quantities of nuclear ribonucleic acidExperimental Cell Research, 1964