Requirement of autophosphorylated tyrosine 992 of EGF receptor and its docking protein phospholipase Cγ1 for membrane ruffle formation

Publisher Website
Google Scholar
Abstract
Stimulation of the epidermal growth factor receptor (EGFR) produces membrane ruffles through the small G protein Rac1; however, the signaling pathway from EGFR to Rac1 has not yet been clarified. Here, we show that autophosphorylation of EGFR at tyrosine 992 is required for EGF-induced membrane ruffle formation in CHO cells. Signaling from the autophosphorylated tyrosine 992 appears to be mediated by phospholipase C (PLC) γ1. Furthermore, activation of Rac1 by EGF is inhibited by a PLC inhibitor. These results, taken together, suggest that autophosphorylation of EGFR at tyrosine 992 and the subsequent PLCγ1 activation transduce the signal to Rac1 to induce membrane ruffle formation